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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
FRET No Wash cAMP Assay Kit
- 供应商：
上海西宝
- 规格：
1 plate
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文献和实验Pasteur, Paris, France Excerpted from Protein: Protein Interactions, Second Edition Edited by Erica A. Golemis and Peter D. Adams ABSTRACT cAMP measurements are obtained using an ELISA assay (Harlow
In Vivo Actin Polymerization ( cAMP)
a. Wash cells with DB and resuspend them to 2 X 107 / ml inDB b. Shake cells at 120 rpm for 1 hour c. Pulse cells with 100 nM cAMP for 4 hours d. Basolate cells with 3 mM caffeine for 15-30 min e. Spin cells down, add DB + 3 mM
an acceptor?sensitized emission FRET method using a confocal microscope for image acquisition. In contrast to acceptor photobleaching, which is an end?point assay that destroys the acceptor fluorophore, the sensitized emission method is amenable for FRET
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