SARS-CoV-2 (2019-nCoV) Spike RBD (K417T, E484K, N501Y) Protein (His Tag)
库存:
99
供应商:
北京义翘神州科技股份有限公司
CAS号:
无
规格:
100 µg
SARS-CoV-2 (2019-nCoV) Spike RBD (K417T, E484K, N501Y) Protein (His Tag)(产品说明)
蛋白名称:新冠病毒Spike RBD (K417T, E484K, N501Y) 重组蛋白 (His Tag)
蛋白构建:A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD (YP_009724390.1, with mutations K417T, E484K, N501Y) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD (K417T, E484K, N501Y) Protein (His Tag) (Cat:40592-V08H86), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD (K417T, E484K, N501Y) Protein (His Tag) (Cat:40592-V08H86) is 3.0-15.0 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Ser
蛋白分子量:The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD consists of 235 amino acids and predicts a molecular mass of 26.7 kDa. As a result of glycosylation, it migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:YP_009724390.1
蛋白氨基酸序列:Arg319-Phe541(K417T, E484K, N501Y)
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.