Mouse CD3D & CD3E Heterodimer Protein(产品说明)
蛋白名称:小鼠 CD3D & CD3E Heterodimer 重组蛋白
蛋白构建:A DNA sequence encoding the extracellular domain (Met1-Ala105) of mouse CD3D (P04235) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp108) of mouse CD3E (P22646) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE.
蛋白活性:检测中
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Phe 22 & Asp 23
蛋白分子量:The recombinant heterodimer of mouse CD3D/CD3E comprises 669 (334+335) amino acids and has a calculated molecular mass of 75.4 (37.5+37.9) kDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 36-55 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:P04235 & P22646
蛋白氨基酸序列:Met1-Ala105 & Met1-Asp108
蛋白标签:C-human IgG1-Fc & His & C-human IgG1-Fc & Flag
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.