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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
上海西宝
- 英文名：
CHEE & POOL C2d VITIS MEDIUM
- 保存条件：
2-6C
- 规格：
10L
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文献和实验Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
实验试剂 Dulbecco’s Modified Eagle Medium (D-MEM) Fetal Bovine Serum (FBS), ESC-Qualified D-MEM/F-12 (1X) with GlutaMAX™-I KnockOut™ Serum Replacement (KSR) MEM Non-Essential Amino Acids Solution, 10 mM
: L-Wnt3A (ATCC , Manassas, VA; ATCC# CRL-2647) (see Note 1 ). 2. Cell culture medium: Dulbecco’s minimum essential medium(DMEM) supplemented with 10% (v/v) fetal bovine serum(FBS), 1:100 dilution of penicillin–streptomycin solution with10,000 U
Purification of GST Fused Proteins
for unstable proteins (room temperature expression can be tried for some insoluble proteins, but the medium should be cooled before induction) Pellet cells in GS3 rotor 5000rpm 10 min at 4°C. Pool cells in small amount of medium and then pellet
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