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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Protein Detector LumiGLO Western Blotting Kit
- 供应商：
上海西宝
- 保存条件：
2-8° C
- 规格：
2500 cm2
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文献和实验SDS-PAGE Western Blotting Protocols
cells or fruiting bodies. Klett 100 cells are at a density of 5E8. 2. Pellet at RT for 1-2’ and remove s/n. 3. Extract protein by SDS/Urea Method, TCA-precipitation, or sonication. 4. Measure [protein] by Bradford (use 1-2 ul of each sample
Protein (Western) Blotting with Immunodetection
Protein blotting simply involves transferring separated protein bands from an acrylamide gel onto a more stable and immobilizing medium, such as nitrocellulose paper. Once transferred to the immobilizing medium, a variety of analytical procedures
with ddwaterPrepare cell extracts using an appropriate protocol. 1. Measure total protein in extract by the Bradford method. Plan on loading approximately 5 μg of protein per lane on the polyacrylamide gel.2. Add an equal volume of SDS sample buffer and boil
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