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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海西宝
- 英文名:
LifeXtend HRP conjugate stabilizer
- 规格:
50ml
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文献和实验Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8oC. Dialyze against 3 changed of PBS, changing every 2 hours, 100X the conjugate
Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8o C
被单克隆或多克隆抗体,这些抗体分别采用以下试剂作为稳定剂:Stabilguard biomolecule stabilizer (SurModics Inc.; Eden Prairie, MN)、 Stabilcoat immunoassay stabilizer (SurModics Inc.)、Superblock blocking buffer (Pierce Chemical Co.; Rockford, IL) 以及牛血清白蛋白 (BSA)。其中使用磷酸盐缓冲液(PBS)作为阴性
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