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- 库存：
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- 供应商：
上海觅拓生物科技有限公司
- 规格：
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文献和实验【求助】topflash/foplash的luciferase opening reading fream针对的就是firefly萤光素酶吗？
lope 请教各位大侠： 购买的UPSTATE的TOPFLASH/FOPFLASH质粒，准备做双荧光素酶报告基因检测，看文献上都只是用这个质粒和Renilla的载体共转染细胞，是否这个质粒本身就携带了Firefly萤光素酶呢？我看了说明书，只是说two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse
Application of the Firefly Luciferase Reporter Gene
” (no radiolabeled compound or organic solvent is used in this assay), and cost efficiency. Here, the properties of the luciferase system are briefly discussed and a simple and rapid protocol used in my laboratory to assay luciferase activity in extract obtained
Improvements in the Application of Firefly Luciferase Assays
The firefly luciferase-based assay differs from most familiar enzyme-based determinations. Most enzyme assays are based either on the production of a product or the disappearance of a substrate. Usually the compound measured is stable
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