Diacylglycerol Kinase Inhibito

HISTONE KINASE ASSAY

C), Roche # 11 017 101 001 Aprotinin 10 mg/mL in PBS (5000-160,000x, aliquot and store -20°C), Roche # 10 981 532 001 PMSF: (phenylmethylsulfonyl flouride, 100x) 0.2 M in ethanol. Store at 4°C. 100x Phosphatase inhibitor mix (store -20ºC

Purification of Myosin Light Chain Kinase

inhibitor* - 8ml of 1mg/ml stock solution pH 7.0 3.Buffer B DE 52 Wash 10 mM MOPS- 20ml of 2M stock solution 4 liters 1 mM EDTA- 20ml of 0.2M stock solution 1 mM DTT* - 4ml of 1M stock solution 0.1 mM PMSF* - 7ml of 10mg/ml stock solution pH

Spatiotemporal Dynamics of Kinase Signaling Visualized by Targeted Reporters

Activator of kinase signaling path: e.g., histamine or phorbol dibutyrate (PdBu) Kinase inhibitor or inhibitor of kinase signaling pathway, as control: e.g., Gö 6983