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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
W IP W IP W IP W IP /
- 供应商:
囊萤科技
- 保存条件:
储存“-80℃”;运输“干燥”
- 规格:
1Kit
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文献和实验that need to be optimized, including the choice of antibody, the conditions for shearing chromatin using sonication and the analysis of immunoprecipitated DNA. The ChIP-IT Kit from Active Motif provides a complete solution to all potential problems associated with the ChIP
Dynabeads® Co-Immunoprecipitation Kit
, although the coupling reaction also works well with antibodies in storage buffers that include protein additives (e.g. BSA) and/or sodium azide (NaN3). This kit is not recommended for use with antibodies that have been stabilized in glycerol. 实验试剂
Active Motif ChIP 实验专家技术经验分享:如何判断 ChIP 成功与否?
通过计算富集度,我们就可以清楚地知道目的蛋白在感兴趣的 DNA 区域的结合强弱。如下图所示:确定 ChIP 成功之后可以将样品建库,测序用于全基因组分析。相关链接:Active Motif 技术专家经验分享:如何才能做好 ChIP 实验?Active Motif ChIP 实验相关对照产品:>> ChIP-IT® Control Kits>> ChIP Control qPCR Primer Sets>> ChIP-IT® qPCR Analysis Kit更多产品详情欢迎咨询 Active
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