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文献和实验实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
15. DPBS Without Calcium or Magnesium 16. TRIzol® LS Reagent 17. SuperScript® VILO™ cDNA Synthesis Kit 18. AccuPrime™ SuperMix I 19. Mouse Anti-Tra1-60 Antibody 20. Mouse Anti-Tra1-81 Antibody 21. Mouse Anti-SSEA
8 for that tube. 10) Resuspend the bead pellet by adding 0.5 mL of buffer to each sample tube. 11) Add one drop of negative beads (Component B in the AbC™ Anti-Mouse Bead Kit) to sample tube(s) containing the AbC™ capture beads. 12) Add
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