新冠病毒SARS-CoV-2 Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His 重组蛋白(产品说明)
蛋白名称:SARS-CoV-2蛋白, SARS-CoV-2 protein
蛋白构建:A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His Recombinant Protein (YP_009724390.1) (Met1-Arg685(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE.
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His (Cat:40591-V08H12), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His (Cat:40591-V08H12) is 10-60 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Val 16
蛋白分子量:The recombinant SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His Recombinant Protein consists of 678 amino acids and predicts a molecular mass of 76.1 kDa. As a result of glycosylation, it migrates as an approximately 112.2 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:YP_009724390.1
蛋白氨基酸序列:Met1-Arg685(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.