- ￥6800
- Mediagnost
- 0.53 – 30000ng/mL
- 0,53ng/mL
- 德国
- E35
- 2026年06月16日
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- 详细信息
- 文献和实验
- 技术资料
- 灵敏度：
0,53ng/mL
- 库存：
10
- 供应商：
广东固康生物科技有限公司
- 检测范围：
0.53 – 30000ng/mL
- 检测方法：
酶联免疫法
- 应用：
——
- 适应物种：
人
- 标记物：
——
- 样本：
血清、血浆
- 规格：
96人份/盒
【产品名称】
通用名称：酸不稳定亚基（ALS）检测试剂盒（酶联免疫法）
英文名称：ALS (Acid Labile Subunit) ELISA
【预期用途】
用于测定人血清或肝素/EDTA/柠檬酸盐血浆中的酸不稳定亚基 (ALS)。仅供科研使用。不可用于临床诊断。
【包装规格】
96T/盒
【背景知识】
参考文献：
1. Ballard.J.. et al.. On the nomenclature of the lGF binding proteins. Acta Endocrinol(Copenh). 1989.121(5): p.751-2.
2. Wilson.E.M..Y.Oh. and R.G. Rosenfeld.Generation and characterization of anIGFBP-7 antibody: identification of 31kD IGFBP-7 in human biological fluids and Hs578Thuman breast cancer conditioned media.J Clin Endocrinol Metab. 1997.82(4): p.1301-3.
3. Baxter. R.C..Characterization of the acid-labile subunit of the growth hormone-dependent insulin-like growth factor binding protein complex.J Clin Endocrinol Metab.1988.67(2): p. 265-72.
4. Baxter.R.C.and J.L. Martin. Structure of the Mr 140.000 growth hormone-dependent'insulin-like growth factor binding protein complex: determination by reconstitution and affinity-labeling. Proc Natl Acad Sci u sA. 1989.86(18): p.6898-902.
5. Holman. S.R.and R.C. Baxter. Insulin-like growth factor binding protein-3: factorsaffecting binary and ternary complex formation. Growth Regul.1996.6(1): p.42-7.
6. Baxter.R.C..Radioimmunoassay for insulin-like growth factor (IGF) ll: interference bypure lGF-binding proteins.J lmmunoassay.1990.11(4): p. 445-58.
7. Blum. w.F. and M.B.Ranke. Use of insulin-like growth factor-binding protein 3 for theevaluation of growth disorders. Horm Res.1990.33 Suppl 4: p.31-7.
8. Miller.B.s.. et al.. The insulin-like growth factor system in children with congenitaldisorders of glycosylation. Clin Endocrinol (Oxf). 2009.
9. Fofanova-Gambetti. O.V.. et al.. Three novel lGFALSgene mutations resulting in totalALS and severe circulating lGF-I/GFBP-3 deficiency in children of different ethnic origins.Horm Res. 2009.71(2): p.100-10.
10. Hwa.V..et al..Total absence of functional acid labile subunit. resulting in severeinsulin-like growth factor deficiency and moderate growth failure.J Clin Endocrinol Metab.2006.91(5): p. 1826-31.
11. Heath. K.E.. et al.. Primary acid-labile subunit deficiency due to recessive lGFALSmutations results in postnatal growth deficit associated with low circulating insulin growthfactor (IGF)-I.lGF binding protein-3 levels, and hyperinsulinemia.J Clin Endocrinol Metab.2008.93(5): p.1616-24.
12. Domene.H.M.. et al..Acid-labile subunit deficiency: phenotypic similarities anddifferences between human and mouse.JEndocrinol lnvest. 2005.28(5 Suppl): p. 43-6.
13.Fischer. F.. et al..Associations of insulin-like growth factors. insulin-like growth factorbinding proteins and acid-Jlabile subunit with coronary heart disease.Clin Endocrinol (Oxf).2004.61(5): p. 595-602.
14. Morrison.K.Mc et al..Sample pre-treatment determines the clinical usefulness of acid-labile subunit immunoassays in the diagnosis of growth hormone deficiency and acromegaly.Eur J Endocrinol.2007,156(3): p.331-9.
15.Tzanela, M..et al. Growth hormone binding protein and acid labile subunit levels inthe assessment of acromegaly treatment. Hormones (Athens).2005.4(3): p. 148-54.
16. Stadler. $et al.. Monoclonal anti-acid-labile subunit oligopeptide antibodies and theiruse in a two-site immunoassay for ALS measurement in humans. J lmmunol Methods. 2001.252(1-2): p.73-82.
17. Khosravi.M.J.. et al.. Acid-labile subunit of human insulin-like growth factor-bindingprotein complex: measurement. molecular.and clinical evaluation.J Clin Endocrinol Metab.1997.82(12): p.3944-51.
通用名称：酸不稳定亚基（ALS）检测试剂盒（酶联免疫法）
英文名称：ALS (Acid Labile Subunit) ELISA
【预期用途】
用于测定人血清或肝素/EDTA/柠檬酸盐血浆中的酸不稳定亚基 (ALS)。仅供科研使用。不可用于临床诊断。
【包装规格】
96T/盒
【背景知识】
血液循环中的胰岛素样生长因子（IGF）-I 与 II，均与特异性结合蛋白（IGFBPs）结合存在。目前已根据氨基酸序列鉴定出 7 种不同的结合蛋白，分别归类为 IGFBP-1 至 IGFBP-7。血液中 IGFBP-3 占主导地位，其主要功能为调控 IGF-I 与 IGF-II 的浓度。
与其他结合蛋白不同，IGFBP-3 与 IGFBP-5 在结合 IGF-I/II 后，可进一步与酸不稳定亚基（acid-labile subunit, ALS）结合，因此 IGFBP-3 在血液中几乎完全以该高分子量三元复合物的形式存在，同时也存在少量游离态 IGFBP-3。
酸不稳定亚基（以下简称 ALS）以含 605 个氨基酸的前肽形式合成，其分泌必需的信号肽（第 1-27 位氨基酸）在转运过程中被切除（Swiss-Prot P35858 版本 82），因此成熟蛋白由 578 个氨基酸组成。ALS 蛋白的特征为富含亮氨酸的序列结构，除 20 个亮氨酸富集序列外，还存在大量 N - 连接糖基化位点。
Miller BS 等研究证实，IGF-I、IGF-II、ALS 与 IGFBP-3 的糖基化不完全，可导致这些蛋白在血清中的浓度降低；口服甘露糖治疗可部分纠正糖基化异常，伴随生长水平改善。ALS 基因突变可导致 IGF-I/IGFBP-3 缺乏，进而引发生长障碍；ALS 完全缺失可导致中度生长障碍。除影响生长障碍外，ALS 缺乏还会影响 IGF-I 及三元复合物的能量代谢，原发性 ALS 缺乏患者可出现高胰岛素血症。此外，ALS-IGF-IGFBP 系统在冠状动脉疾病中也具有重要研究价值。
本检测结果可作为 IGF-I、IGFBP-3 检测的补充，用于辅助评估生长激素缺乏症与生长激素过量状态。
Baxter RC 于 1990 年开发了 ALS 的自建放射免疫检测法，证实健康人血清中 ALS 浓度可达 50 μg/mL；羊水、脑脊液、精浆中虽含有高浓度 IGFBP-3，但未检测到 ALS。
参考文献：
1. Ballard.J.. et al.. On the nomenclature of the lGF binding proteins. Acta Endocrinol(Copenh). 1989.121(5): p.751-2.
2. Wilson.E.M..Y.Oh. and R.G. Rosenfeld.Generation and characterization of anIGFBP-7 antibody: identification of 31kD IGFBP-7 in human biological fluids and Hs578Thuman breast cancer conditioned media.J Clin Endocrinol Metab. 1997.82(4): p.1301-3.
3. Baxter. R.C..Characterization of the acid-labile subunit of the growth hormone-dependent insulin-like growth factor binding protein complex.J Clin Endocrinol Metab.1988.67(2): p. 265-72.
4. Baxter.R.C.and J.L. Martin. Structure of the Mr 140.000 growth hormone-dependent'insulin-like growth factor binding protein complex: determination by reconstitution and affinity-labeling. Proc Natl Acad Sci u sA. 1989.86(18): p.6898-902.
5. Holman. S.R.and R.C. Baxter. Insulin-like growth factor binding protein-3: factorsaffecting binary and ternary complex formation. Growth Regul.1996.6(1): p.42-7.
6. Baxter.R.C..Radioimmunoassay for insulin-like growth factor (IGF) ll: interference bypure lGF-binding proteins.J lmmunoassay.1990.11(4): p. 445-58.
7. Blum. w.F. and M.B.Ranke. Use of insulin-like growth factor-binding protein 3 for theevaluation of growth disorders. Horm Res.1990.33 Suppl 4: p.31-7.
8. Miller.B.s.. et al.. The insulin-like growth factor system in children with congenitaldisorders of glycosylation. Clin Endocrinol (Oxf). 2009.
9. Fofanova-Gambetti. O.V.. et al.. Three novel lGFALSgene mutations resulting in totalALS and severe circulating lGF-I/GFBP-3 deficiency in children of different ethnic origins.Horm Res. 2009.71(2): p.100-10.
10. Hwa.V..et al..Total absence of functional acid labile subunit. resulting in severeinsulin-like growth factor deficiency and moderate growth failure.J Clin Endocrinol Metab.2006.91(5): p. 1826-31.
11. Heath. K.E.. et al.. Primary acid-labile subunit deficiency due to recessive lGFALSmutations results in postnatal growth deficit associated with low circulating insulin growthfactor (IGF)-I.lGF binding protein-3 levels, and hyperinsulinemia.J Clin Endocrinol Metab.2008.93(5): p.1616-24.
12. Domene.H.M.. et al..Acid-labile subunit deficiency: phenotypic similarities anddifferences between human and mouse.JEndocrinol lnvest. 2005.28(5 Suppl): p. 43-6.
13.Fischer. F.. et al..Associations of insulin-like growth factors. insulin-like growth factorbinding proteins and acid-Jlabile subunit with coronary heart disease.Clin Endocrinol (Oxf).2004.61(5): p. 595-602.
14. Morrison.K.Mc et al..Sample pre-treatment determines the clinical usefulness of acid-labile subunit immunoassays in the diagnosis of growth hormone deficiency and acromegaly.Eur J Endocrinol.2007,156(3): p.331-9.
15.Tzanela, M..et al. Growth hormone binding protein and acid labile subunit levels inthe assessment of acromegaly treatment. Hormones (Athens).2005.4(3): p. 148-54.
16. Stadler. $et al.. Monoclonal anti-acid-labile subunit oligopeptide antibodies and theiruse in a two-site immunoassay for ALS measurement in humans. J lmmunol Methods. 2001.252(1-2): p.73-82.
17. Khosravi.M.J.. et al.. Acid-labile subunit of human insulin-like growth factor-bindingprotein complex: measurement. molecular.and clinical evaluation.J Clin Endocrinol Metab.1997.82(12): p.3944-51.
注意
本产品仅供科研使用。不可用于诊断或治疗程序。
德国mediagnost（美狄洛斯）中国官方代理商：广东固康生物科技有限公司
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文献和实验相关实验
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美狄洛斯®酸不稳定亚基（ALS）检测试剂盒（酶联免疫法）
￥6800
