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- S7903
- 2025年07月14日
- PCR
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
PCR
- 宿主：
0
- 库存：
大量
- 抗原来源：
0
- 是否单克隆：
0
- 规格：
10000 assays
Description:
Amplifluor® UniPrimer II Fluorescein
Trade Name:
Chemicon (Millipore)
Product Overview:
The Amplifluor™ UniPrimer™-fluorescein is a hairpin primer labeled with a fluorescent energy transfer pair (fluorescein and non-fluorescent quencher) and a 3' single-stranded oligonucleotide tail and is provided at a concentration of 5.0 μM (25.0 mL).
Fluorescence (Linear:Hairpin): 39. Excitation = 495nm; Emission = 516nm.
Fluorescence (Linear:Hairpin): 39. Excitation = 495nm; Emission = 516nm.
Key Applications:
PCR
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Reagent Type:
PCR Reagents
Kit or Assay Type:
ELISA Kits
Presentation:
5.0μM (25.0mL)
Storage Conditions:
Minimize exposure to light as this material is light sensitive. Make sterile aliquots of bulk solution. Store long term at -65°C to -75°C. Packaged product for end user may be stored in the dark at -15°C to -25°C.
Recommended diluent: autoclaved, reagent grade, deionized water only.
Recommended diluent: autoclaved, reagent grade, deionized water only.
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
详细描述见链接：http://www.millipore.com/catalogue/item/S7903
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文献和实验相关实验
的同聚核苷酸锚定序列。利用第二个巢式GSP和一个与同聚核苷酸尾巴可以退火的锚定引物来扩增cDNA的5＇末端。用SUPERSCRIPTTM II RT从mRNA复制成cDNA用RNase H和RNase T1降解RNA用GLASSMAXò Spin Cartridge纯化cDNA用dCTP和TdT给纯化的cDNA加尾用精简的锚定引物和巢式GSP2 PCR扩增dC加尾的cDNA 用AUAP和巢式GSP重新扩增初步PCR产物最初的5＇RACE系统是按照这个方法进行的，它首先利用SUPERSCRIPT™
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密理博 Amplifluor UniPrimer II荧光引物 PCR用 荧光标记引物对pcr
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