DAPI/Antifade Solution, Ready

DAPI/Antifade Solution, Ready to Use

Chemicon (Millipore)

DAPI: 4', 6-Diamino-2-phenylindole dihydrochloride. Antifade Solution: p-Phenylenediamine and glycerol in PBS solution. 0.4μg/mL DAPI suspended in Antifade. The product is ready to add to fixed slides or cells cultures.

A nuclear fluorescent stain used for counterstaining paraffin-embedded, formalin-fixed tissue, metaphase chromosomes, or interphase nuclei that have been hybridized with probes detected with Fluorescein, or equivalent stains. Antifade prevents the permanent loss of fluorescence or fluorescent signal quenching due to photobleaching and prolonged exposure to high intensity light sources.

DAPI binds the minor groove of DNA at AT clusters and binds preferentially to ds DNA (Biochemistry 26:4545, 1987).

Materials Provided

Each vial contains 1 ml of DAPI/Antifade Solution

Warnings and Precautions

DAPI is a potential carcinogen. Antifade is a potential toxin and irritant. Use extreme caution. Avoid contact with eyes, skin and mucous membranes. Use extreme caution. Wear gloves and a lab coat when handling. Follow your institution's guidelines for hazardous waste disposal. Keep tightly capped and limit exposure to light.



Related Products

Antifade Solution S7114

Propidium Iodide Solution S7109

Propidium Iodide/Antifade Solution S7112

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

• Apoptosis Assays
• Apoptosis Assays

Liquid PBS pH 8.0 containing ~50% glycerol and 0.4μg/mL of DAPI and 2-7mM phenylenediamine.

Store at -20ºC in undiluted aliquots for up to 1 year from date of receipt. Protect from light

Fluorescent

• Detection Reagents
• Immunohistochemistry Reagents