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- S-014-C
- 2025年07月13日
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100 mL
Enzyme-Free dissociation solution should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, we recommend trypsinizing the cells and replating at 30-40% confluency 24 hours before enzyme-free use (this will allow the cells to be 60-80% confluent for enzyme free dissociation).
- Mouse Embryonic Stem Cells
- Human Embryonic Stem Cells
- Mesenchymal Stem Cells
- Neural Stem Cells
- Hematopoietic Stem Cells
- Epithelial Cells
- Pancreatic Stem Cells
- Cardiac Stem Cells
- Induced Pluripotent Stem Cells
- Cell Culture Dissociation Reagents
- Cell Culture Supplements
- Stem Cell Reagents
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
详细描述见链接：http://www.millipore.com/catalogue/item/S-014-C
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文献和实验Protein Syntheses in Cell Free Systems
/v) glycerol 1X TBS-M buffer containing 0.25 M sucrose 1X TBS-M buffer containing 1.0 M sucrose Sephadex G-25 column equilibrated with 1X TBS-M buffer Liquid nitrogen storage Reaction mixture for protein synthesis
Neuronal Cell Dissociation Protocol
• To 250 ml of 0.2M PBS add: 43.8 g NaCl 1 g KCl • Mix well and add dH2O to a final volume of 500 ml. 4. Dissociation solutions • Label three 50 ml
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
once with 2 mL of 1X PBS (Ca2+ /Mg2+ -free) per well. 10. Replace the PBS with 1 mL of collagenase solution and incubate for 10-15 min at 37°C. Incubation time will vary depending on the batch of collagenase solution and the hESC lines
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