PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated (See ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:
6 x 60mm dishes
2 x T75's
2 x 6 well plates
See ES Stem Cell Manual for complete culture and media for PMEF cells
Key Applications:
Stem Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Stem Cell Type:
Human Embryonic Stem Cells
Mouse Embryonic Stem Cells
PMEFs
Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Cells/Isolation
Maintenance & Expansion
Cell Line Type:
Primary Mouse Embryo Fibroblasts
Storage Conditions:
After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
Packaging:
5-6x106 ea
Cell Culture Reagent Type:
Cell Culture Supplements
Stem Cell Reagents
Alternate Names:
Non drug resistant cells from CF-1 mice- Mitomycin C treated