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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海博湖
- 检测范围:
0.156-10ng/ml
- 检测方法:
Sandwich ELISA, Double Antibody
- 应用:
0.094ng/ml
- 样本:
HNRPU
- 规格:
96T
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文献和实验at 4°C. Transfer supernatant (nuclear fraction) to a clean microcentrifuge tube. 11. Aliquot and store at –80°C. The nuclear extracts are ready for assay. 12. Quantitate protein concentration using the Quant-iT™ Protein Quantitation Kit (Cat. no. Q
Activation and Expansion of Human Treg Cells
from the magnet. (5)Resuspend the washed Dynabeads in the same volume of Buffer 1 as the initial volume of Dynabeads. 2. Isolation of Human Treg Cells For isolation of human Treg cells it is recommended to use the Dynal CD4 CD25 Treg Kit. 3. Expansion
: 10 mM each dATP, dCTP, dGPT, and dTTP (unit 3.4 ) 13 U/µl Sequenase (USB) stock: dilute 1/10 immediately before use 10
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