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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海博湖
- 检测范围:
7.813-500ng/ml
- 检测方法:
Sandwich ELISA, Double Antibody
- 应用:
4.688ng/ml
- 样本:
C3d
- 规格:
96T
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文献和实验C3d的定量检测采用ELISA双抗体夹心法,其原理为抗C3d血清能与C3、C3b、C3bi发生交叉反应,根据C3SP与C3在不同浓度PEG中溶解度的不同,用11%PEG溶解待测样本中C3d,然后加至包被抗C3d反应板中,再依次加入HRP标记的抗C3d抗体和底物OPD/H202,用4mol/L H2S04终止反应,于492nm读取吸光度,C3d含量与其吸光度呈正相关。 1、10mmol/LEDTA抗凝正常人或病人全血,2500rpm离心10min,取血浆置-70℃保存。 2、C3d标准
Evaluation of Complement Function by ELISA
Evaluation of total complement function in human serum is an essential component of laboratory diagnostics of the human complement system. During recent years, established hemolytic assays for classical pathway and alternative pathway
by a two- or three-step procedure. The method can insert any fragment into small or large episomes, without the need of an antibiotic selection gene. We have humanized the mouse luteinizing hormone receptor gene (Lhcgr ) by inserting a ~55 kb fragment from a BAC clone
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