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- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
零下80~20°C
- 保质期：
Powder: -20°C for 3 years In solvent: -80°C for 2 years
- 英文名：
12-Ethyl-9-hydroxycamptothecin
- 库存：
10
- 供应商：
TargetMol
- CAS号：
119577-28-5
- 规格：
5 mg
Product Introduction
Bioactivity
英文名:12-Ethyl-9-hydroxycamptothecin
描述:12-Ethyl-9-hydroxycamptothecin is a natural product isolated from Camptotheca acuminata Decne
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文献和实验two times with 500 µl of ice-cold washing buffer. 7. vortex the pellet and centrifuged for 5 seconds at 12,000 RPM. 8. Remove the buffer and wash the pellet one to two times with 100% ethyl alcohol . 9. Remove ethyl alcohol and dry
the DNA with equal volumes of buffered phenol, Phenol-chloroform- isoamyl alcohol (v/v, 25:24:1) and finally chloroform-isoamyl alcohol (v/v, 24:1). 5. precipitate the DNA with 3-M ammonium acetate and 2.5 volume of 100% ethyl alcohol
for 2-3'. 7. Resuspend in 100 µl 0.25M Tris pH 7.8 8. Freeze-thaw 3x (10'freeze-10'thaw). 9. Spin down debris @14K RPM for 5'. 10. Transfer 100 µl supernatant to a new set of tubes. 11. Set new set of tubes and add 40 µl of supernatant to each. 12. In separate
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