- ￥530
- Biorigin
- 北京
- BN20742
- 2025年11月12日
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Biorigin
- 规格：
50ml
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文献和实验RNase Protection Assay (Bowtell Lab)
0.5ul 200mM DTT 0.5ul appropriate RNA polymerase Incubate for 90-120min at 41�. 5. Add 0.5ul RNAsin and 0.5ul RNAse free DNAse (5mg/ml). Incubate for 15min at 37�. 6. Add 10ug tRNA and 100ul DDW. Phenol/CHCl3 extract and precipitate with 50ul 5M NH
can then be used as a template for synthesis or radiolabeled anti-sense RNA probes. Standard RPA Procedure In all steps of the protocol, standard precautions should be used to avoid RNase contamination and exposure of personnel to radioactivity. Typically
RNase and DEPC Treatment: Fact or Laboratory Myth
. FALSE, but.. . The amount of DEPC required to inactivate RNase increases as the amount of contaminating RNase in a solution increases (Figure 3). 100, 500, and 1000 ng/ml RNase A was added to water followed by various amounts of DEPC
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