- 询价
- MAB318
- 2025年07月03日
- Immunoprecipitation;Western Blotting
- Mouse
- Chicken;Human;Mouse;Monkey
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 应用范围：
Immunoprecipitation;Western Blotting
- 宿主：
Mouse
- 库存：
大量
- 抗原来源：
0
- 适应物种：
Chicken;Human;Mouse;Monkey
- 是否单克隆：
1
- 规格：
100 μL
Mouse anti-Tyrosine Hydroxylase (Catalog Number MAB318) Staining of rat dorsal root ganglia (DRG) after sciatic nerve transection for 2 weeks. Staining shows sympathetic nerve fibers sprouting into DRG.
Mouse anti-Tyrosine Hydroxylase (Catalog Number MAB318) staining of embryonic rat mesencephalic dopaminergic neurons
Rabbit anti-DARPP-32 (Catalog Number AB1656). Localization of DARPP-32 (green) and Tyrosine Hydroylase (red) (Catalog Number MAB318) in cultured primary neurons. Photo courtesy of Dr. Mehdi Doroudchi, Avigen.
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, Fg, H, M, Mk, R, Lz, Vo, Zebrafish | IH, IH(P), IP, WB | Mouse | Ascites | Monoclonal Antibody |
- TH
- Tyrosine Monooxygenase
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Immunoprecipitation
- Western Blotting
A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.
Immunoprecipitation:
A previous lot of this antibody was used in IP (Perez, 2002).
Optimal working dilutions and protocols must be determined by end user.
- Chicken
- Frog
- Human
- Mouse
- Monkey
- Rat
- Lizard
- Vole
- Zebrafish
- TH
- TYH
- EC 1.14.16.2
Western blot:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.
- Neurotransmitters & Receptors
- Neuronal & Glial Markers
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Tyrosine Hydroxylase purified from PC12 cells. 详细描述见链接：http://www.millipore.com/catalogue/item/MAB318
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文献和实验Phenylalanine Hydroxylase, Tyrosine Hydroxylase, and Tryptophan Hydroxylase
There are four enzymes that utilize reduced pteridine as an electron donor and incorporate one atom of oxygen into their substrates. They are phenylalanine 4-monooxygenase (phenylalanine hydroxylase, PAH, EC 1.14.16.l), tyrosine 3-monooxygenase
Clone Genes From a Phage Library
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MEDIUM FOR EMBRYO RESCUE (FOR 1 L)
Organic constituents ucrose (6 % final concentration) � 30 g i-inositol (100 mg/L stock is 0.5 g/50 mL) � 10 mL Thiamine (0.4 g/l stock is 2 g/50 mL) � 10 mL Amino acids L-tyrosine, L-
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