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Anti-Macrophages/Monocytes, cl

one ED-1; 100 μg
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  • 询价
  • MAB1435
  • 2025年07月12日
  • Flow Cytometry;Immunoprecipitation
  • Mouse
  • Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      Flow Cytometry;Immunoprecipitation

    • 宿主

      Mouse

    • 抗原来源

      0

    • 库存

      大量

    • 适应物种

      Rat

    • 是否单克隆

      1

    • 规格

      100 μg

    Species Reactivity Key Applications Host Format Antibody Type
    R FC, IC, IH, IP, RIA, WB Mouse Purified Monoclonal Antibody
    Description:
    Anti-Macrophages/Monocytes, clone ED-1
    Replaces:
    CBL1511
    Background Information:
    Monocytes are produced by the bone marrow from haematopoietic stem cell precursors called monoblasts. Monocytes circulate in the bloodstream for about one to three days and then typically move into tissues throughout the body. They consist of between 3 to 8 percent of the leukocytes in the blood. In the tissues monocytes mature into different types of macrophages at different anatomical locations.
    Macrophages are a type of white blood cell derived from monocytes that engulf invading antigenic molecules, viruses, and microorganisms and then display fragments of the antigen to activate helper T cells; ultimately stimulating the production of antibodies against the antigen.
    Clone:
    ED-1
    Specificity:
    Recognizes a single chain glycoprotein of 90-100 kD that is expressed on the lysomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen has many characteristics in common with macrosialin in the mouse and CD68 in the human.
    Key Applications:
    • Flow Cytometry
    • Immunocytochemistry
    • Immunohistochemistry
    • Immunoprecipitation
    • Radioimmunoassay
    • Western Blotting
    Application Notes:
    Immunohistology on frozen sections at 1:50-1:300. Paraffin embedded tissue (Bouin fixation): 1:50-1:300 (requires pretreatment with trypsin or pronase).

    Flow cytometry at 1:100 - 1:200. Use 10 μL of this working dilution to label 10E6 cells.

    Radioimmunoassay

    Also suitable for Immunoprecipitation, and Western Blotting.

    Optimal working dilutions must be determined by end user.
    Species Reactivity:
    Rat
    Isotype:
    IgG1
    Immunogen:
    Rat spleen cells
    Molecular Weight:
    90-100 kD
    Control:
    Macrophages, monocytes, lymphoid organs
    Presentation:
    Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
    Storage Conditions:
    Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    Antibody Category:
    Inflammation & Immunology
    Antibody Sub-Category:
    Immunoglobulins & Immunology
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    Purified
    Host:
    Mouse
    Concentration:
    1 mg/mL
    Antibody Type:
    Monoclonal Antibody

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    Rat spleen cells 详细描述见链接:http://www.millipore.com/catalogue/item/MAB1435

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