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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Flow Cytometry;Immunoprecipitation
- 宿主:
Mouse
- 抗原来源:
0
- 库存:
大量
- 适应物种:
Rat
- 是否单克隆:
1
- 规格:
100 μg
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | FC, IC, IH, IP, RIA, WB | Mouse | Purified | Monoclonal Antibody |
Macrophages are a type of white blood cell derived from monocytes that engulf invading antigenic molecules, viruses, and microorganisms and then display fragments of the antigen to activate helper T cells; ultimately stimulating the production of antibodies against the antigen.
- Flow Cytometry
- Immunocytochemistry
- Immunohistochemistry
- Immunoprecipitation
- Radioimmunoassay
- Western Blotting
Flow cytometry at 1:100 - 1:200. Use 10 μL of this working dilution to label 10E6 cells.
Radioimmunoassay
Also suitable for Immunoprecipitation, and Western Blotting.
Optimal working dilutions must be determined by end user.
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
Rat spleen cells 详细描述见链接:http://www.millipore.com/catalogue/item/MAB1435
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文献和实验107 的 PBMC 细胞用 100μl 的 Buffer 稀释(Buffer CI:含 0.5% BSA 的 PBS 缓冲液,1 mM EDTA) · 加入多种生物素化抗体,室温下孵育 5 分钟。生物素化的抗体包含以下几种,每种抗体 0.5 μg:CD235a (erythrocytes), CD14 (monocytes), CD3 (T cells), CD15 (granulocytes), CD19 (B cells), CD36 (thrombocytes), CD123
of Hydroquinone, a Benzene Metabolite, on In Vitro Inflammatory Responses Mediated by Macrophages, Monocytes, and LymphocytesJae Youl Cho*School of Bioscience and Biotechnology and the Institute of Bioscience and Biotechnology, Kangwon National University
on a 3" petri plate, the maximum practical density to allow isolation of individual colonies is about 100-200 colonies per plate. If we were to try to plate our library of 3.37 x 106 in such a way would need about 22,500 plates .
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