- 询价
- MAB1406
- 2025年07月15日
- Flow Cytometry;Immunocytochemistry
- Mouse
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Flow Cytometry;Immunocytochemistry
- 宿主：
Mouse
- 抗原来源：
0
- 库存：
大量
- 适应物种：
Rat
- 是否单克隆：
1
- 规格：
100 μg
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | FC, IC, IH | Mouse | Purified | Monoclonal Antibody |
- Flow Cytometry
- Immunocytochemistry
- Immunohistochemistry
Immunohistochemistry on frozen sections.
Immunocytochemistry
Optimal working dilutions must be determined by end user.
- THY1
- CD90
- CDw90
- FLJ33325
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Rat Thy-1 详细描述见链接：http://www.millipore.com/catalogue/item/MAB1406
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文献和实验policeman.4. Put the extract into an eppendorf and boil for 5 min., cool on ice.5. Load onto 10% SDS-PAGE. I usually load about 1/5 of the lysate from a 60 mm dish. This is about 100 μg protein for PC12 cells.6. After electrophoresis, electrotransfer
Dual- and Triple-Color Fluorospot
stimulatory agents of choice and anti-CD28 (0.1 μg/ml; seeNote 4), in a final volume of 100–150 μl/well. The number of cells per well is dependent on the stimulating agent used (seeNote 5). b. Incubate the plate overnight at 37°C in a humidified
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
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