PickMutant™ Random Mutagenesis

Localized Random Polymerase Chain Reaction Mutagenesis

(2 ,3 ). Until recently, surprisingly little attention has been paid to application of PCR as a tool for localized random mutagenesis. Taq DNA polymerase (from Thermus aquaticus ), the most commonly used thermal stable DNA polymerase in PCR, exhibits a relatively high

SMART™ cDNA 技术问答

有SMART cDNA 合成和文库构建到? TriplEx2 或者Creator 供体载体 pDNR-LIB所需的试剂。因为该试剂盒是特别针对文库构建来设计的，所以它与Clontech™ PCR-Select™cDNA 差减杂交试剂盒不能配合使用。 SMART PCR cDNA Synthesis Kit 可以用来与Clontech™ PCR-Select™ cDNA 差减技术一起使用。总RNA 不能直接用来做差减实验，但可以通过SMART PCR cDNA Synthesis Kit 来扩增出

Mutagen: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases

Random mutagenesis is one of the most effective methodologies to generate variant libraries for directed protein evolution. Indeed, this approach requires no structural or mechanistic information and can uncover unexpected beneficial