HKOH-1,2031170-96-2

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HKOH-1

CAS No. : 2031170-96-2

MCE 国际站：HKOH-1

产品活性：HKOH-1 是一种特异性检测活细胞内 ·OH 的高灵敏度绿色荧光探针，最大激发波长和发射波长分别为 500 nm 和 520 nm。

研究领域：Immunology/Inflammation  |  NF-κB  |  Metabolic Enzyme/Protease  |  Others

作用靶点：Reactive Oxygen Species  |  Fluorescent Dye

In Vitro: 1. Preparation of HKOH-1 working solution
1.1 Preparation of the stock solution
Dissolve 1 mg HKOH-1 in 135 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃ -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of HKOH-1 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of HKOH-1 working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

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