MCE 2× Fast PCR Master Mix (with Dye) is a ready-to-use premix for rapid PCR amplification. The product contains modified Taq DNA Polymerase, extension-enhancement factor, dNTPs Mix, MgCl2 and optimized reaction buffer. The amplification speed is up to 15 sec/kb and the maximum amplification speed is 5 sec/kb within 1 kb. With the primers and template added, the optimized system will provide sensitive and reliable DNA synthesis. After the PCR reaction, the PCR products can be loaded directly onto an agarose gel. It saves preparation time, reduces the risk of contamination from multiple pipetting steps, and provides consistent reaction-to-reaction performance. The PCR products contain poly(A) at the 3'-end and can be directly cloned into T-Vectors.