Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
Key Applications:
Radioimmunoassay
Application Notes:
Overnight, equilibrium incubation at 4°C. Sample volume 100 μL serum, plasma, or tissue culture media.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kit or Assay Type:
Endocrine Kits
Radioimmunoassays (RIA)
Therapeutic Areas:
Inflammation
Linearity of Dilution:
88–101%
Label:
125I-Human Leptin
Precision, %:
Inter-assay: 3.6–6.2
Intra-assay: 3.4–8.3
Accuracy:
103–105%
Detection Methods:
Radioactive
125I
Gene Symbol:
LEP
leptin
OB
obesity
OBS
Performance:
ED80 = 0.9 ± 0.1 ng/ml
ED50 = 4.5 ± 0.4 ng/ml
ED20 = 22.2 ± 2.8 ng/ml
Standard Curve Range:
0.5–100 ng/mL
Specificity:
Human Leptin 100% Rat Leptin <0.2% Mouse Leptin <0.2% Human Insulin Not Detectable Human C-Peptide Not Detectable Glucagon, IGF-I Not Detectable Somatostatin, PP Not Detectable Rat Insulin Not Detectable Human Proinsulin Not Detectable Canine Lep