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上海玉博生物科技有限公司
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文献和实验should be titrated for optimal dilution) Add 100uL of second step antibody to wells and incubate for 1hr Repeat washing step Prepare substrate solution* Add 100uL of substrate to well and incubate at RT for 60min add stopping
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones. Procedure 1. All incubations
4) ABTS Liquid Substrate: Aspirate and wash plate 4 times. Add 100μl of substrate solution to each well. Incubate at room temperature for color development. Monitor color development with an ELISA plate reader at 405 nm with wavelength correction set
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