- ￥3000
- invitrogen
- 026502
- 2025年07月14日
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上海玉博生物科技有限公司
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文献和实验as many as 6 million dpm to maximize sensitivity. 4) Put the 100,000 dpm samples aside until step #12. 5) After lyophilization, solubilize 200,000 dpm samples in 350 µl IMP buffer. 6) Add 50 µl of antibody or control (normal rabbit serum, mouse isotype control
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
of antibody or control (normal rabbit serum, mouse isotype control,, etc.) to each tube and incubate overnight for 6 h at room temperature. 7) Add 100 µl of Protein-A-Sepharose solution (shake to suspend slurry before pipetting) and incubate on a tube
Flow Cytometric Analysis Of Bcl Family members
minutes to prevent non-specific antibody binding. Cells were washed thrice in FACS buffer, incubated with the relevant antibody or relevant isotype matched control antibodies at 4„aC for 40 minutes. This was followed by three washes in FACS buffer
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