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文献和实验Bicinchoninic Acid (BCA) Protein Assay (Smith)
)has suggested that the BCA assay will replace the Lowry because it requires a single step,and the color reagent is stable under alkaline conditions. Both a standard assay for concentrated proteins and a micro assay for dilute protein solutions are described
Quantitation of Protein:Bicinchoninic Acid (BCA) Method
CuSO 4 ·5H 2 O (4%), add distilled water to 50ml. ③ BCA Reagent: Take 50 allots Reagent A and 1 allots Reagent B, mix up. This reagent can be stable for a week. (2) Standard protein solution: Weigh 40mg bovine serum albumin
)].Under alkaline conditions,copper complexes with protein.When folin phenol reagent (phospho-molybdic-phosphotungstic reagent)is added,the Folin-phenol reagent binds to the protein.Bound reagent is slowly reduced and changes color from yellow to blue.While widely
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