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文献和实验Two-hybrid analysis of genetic
mating type come into contact,and results in fusion of the two haploids to form a diploid yeast strain.Thus,an interaction can be determined by measuring activation of a two-hybrid reporter gene in the diploid strain.As described below,interaction mating
Preparing Colloidal Gold for Electron Microscopy
Preparation of Colloidal Gold Conjugates. Colloidal gold has been used for centuries in the preparation of stained glass for windows and fine glassware. In recent years, colloidal
Yeast Transformation inoculate 5-10 ml culture with single colony and grow ON measure OD600nm and dilute to OD600nm = 0.3 in 50 ml grow for 2-4 hrs spin, wash cells with 25 ml H2 O spin
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