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上海玉博生物科技有限公司
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文献和实验histone PtK1s 1) rinse cylinder well with ddH2 O 2) add 600 mls ddH2 O 3) add one bottle MEM (Sigma #M-0643) 4) add 10uM Hepes 5) pH to 7.2 with 1N NaOH 6) bring up to 900 mls with ddH2 O-test pH 7) add 5 mLs of NEAA (non-essential
fungizone, antibiotics (pen and strep) and FBS to one label complete DMEM 10) just add media to other and label incomplete DMEM L-15 for PtK1s 1) rinse cylinder well with ddH2 O 2) add 600 mls ddH2 O 3) add 1 bottle L-15 (Sigma #L-4386
DDW 0.5 mL 2 mL 5 mg/mL cholesterol in 95% EtOH Warm to 37°C O/N to dissolve. When this is added to an aqueous solution some cholesterol will precipitate; don't worry about this. Aliquot to four 2L flasks
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