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上海玉博生物科技有限公司
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文献和实验water:无Rnase的水。方法是:将DEPC按0.01%(V/V)加在dH2O中500 ml(50 ul),在37 ℃过夜,并高压灭菌即得(150 ℃ 3小时) 5. 一次性塑料手套 6. 注意:DEPC有致癌之嫌 7. 抽提出的细胞总RNA干燥后加水50 ul,取10 ul加无Rnase水990 ul,稀释100倍成1 ml。于0.5 cm厚的石英比色杯中,以无Rnase水为对照,在721型紫外分光光度计下检测,结果应为:A260/280 ratio 8. 分离组织10 mg
Avoiding DNA Contamination in RT-PCR
. In addition to DNA-free , Ambion offers many quality products to facilitate successful RT-PCR experiments. These include RNase-free pipette tips and PCR tubes, RNase free DNase I, ToTALLY RNA, RNAqueous, and Poly(A)Pure RNA Isolation Kits
7b. Pool the supernatants from step 6 into 500 ml bottles and add DNase-free RNase A and RNase T1 such that the final concentration of RNase A is 40 ug/ml and RNase T1 is 40 U/ml. Incubate in a 37degC water bath for 30 minutes. 8b. Add an equal
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