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文献和实验Preparation of Luciferin for In Vitro and In Vivo Bioluminescent Assays
Preparation of Luciferin for In Vitro Bioluminescent Assays Materials • D-Luciferin Firefly, potassium salt, 1.0 g /vial (Caliper Life Sciences Part Number #119222) • Sterile water • Complete media Procedure 1. Prepare a 200
In Vivo Luciferin Imaging Procedure
Bioluminescent Assays Materials • D-Luciferin, Firefly, potassium salt, 1.0 g/vial, (Caliper Life Sciences Part Number #119222) DPBS, w/o Mg2+ and Ca2+ Note: One can either reconstitute the entire 1.0 g of D Luciferin in 66.6 mL of DPBS or reconstitute
Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
prostaglandin F α ‐d 4 ; Cayman Chemical cat. no. 316350) in methyl acetate 1 mg/ml 15 F 2t ‐isoprostane (8‐iso prostaglandin F α ; Cayman Chemical cat. no. 16350) in absolute ethanol
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