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文献和实验of a master mix containing water, buffer, dNTPs, primers and Taq DNA Polymerase in a single tube, which can then be aliquoted into individual tubes. MgCl2 and template DNA solutions are then added. This method of setting reactions minimizes the possibility
, temperatures, and amount of template DNA, may vary and must be individually determined. 1) Thaw primer solutions. Keep on ice after complete thawing, and mix well before use. 2) Mix the PCR Master Mix by vortexing briefly. It is important
SequalPrep Long PCR Kit with dNTPs
μl of master mix into each PCR tube/well, and add: Primer mix (10 μM each) 1 μl 0.5 μM each Template DNA (1–100 ng/μl) 1 μl as required 4. Cap the tube/well, tap
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