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Transformation of E. coli by Electroporation

for electroporation. 1. Use a fresh colony of DH5α (or other appropriate host strain) to inoculate 5 ml of SOB (without magnesium) medium in a 50 ml sterile conical tube. Grow cells with vigorous aeration overnight at 37EC. 2. Dilute 2.5 ml of cells into 250

Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis

the bottom of the syringe with Parafilm and place in a 17 x 100 mm polypropylene tube. 8. Add 0.5 ml Crush and Soak Buffer to the gel fragments and cover the top of the tube with Parafilm. 9. Incubate at 37°C for 2 to 3 hr with shaking on platform

MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE

  Media Used To prepare 100 ml medium DMEM 80 ml FCS 15 ml Non-essential amino acids (100x) 1 ml Pen/strep (5,000 1U/ml, 5000 ug/ml