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文献和实验How do I decontaminate my tissue culture
to save the culture with the following procedure. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose response test to determine the level at which an antibiotic or antimycotic becomes toxic
How do I decontaminate my tissue culture
with the following procedure. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important
Method: Removal of Yeast Contamination from Lymphoblast Cultures
media: To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add: 165.0 ml fetal bovine serum, heat inactivated 12.0 ml 200mM (100X) L-glutamine 12.0 ml antimycotic/antibiotic (100X), liquid, Gibco, Cat. No. 600-5240AG Filter sterilize
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