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上海玉博生物科技有限公司
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
a 200 μL pipette, transfer the cut pieces to a freshly prepared 24-well MEF culture plate containing human iPSC medium. 6) Incubate the MEF culture plate containing the picked colonies in a 37°C incubator with a humidified atmosphere of 5% CO2
LIVE/DEAD® Violet Viability/Vitality Kit
5 vials, 25 μg each ≤–20°C Desiccate Protect from light When stored as directed, kit components are stable for at least 6 months
Activation and Expansion of Human Treg Cells
1: PBS w/ 0.1% BSA, pH 7.4 Cultural Medium: OpTmizer? T-Cell Expansion SFM (Gibco) serum free 1X formulation designed to support the culture and expansion of human T cells(or equivalent) Round bottom tissue culture plates or tissue culture flasks
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