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3-Hydroxy-Fatty Acid Analysis by Gas Chromatography-Mass Spectrometry

the functioning of organs and systems whose high-energy requirements utilize fats for a continuous energy source, such as heart and skeletal muscle. This method quantitatively measures intermediate metabolites of fatty acid β-oxidation, specifically the 3-hydroxy

4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody

, and can also attack DNA or protein (1 ). During the process of lipid peroxidation, polyunsaturated fatty acids (PUFA), especially linoliec acid, arachidonic acid, and docosahexaenoic acid, in biomembranes are degraded to a great variety of water-soluble, short-chain

Site-directed hydroxy radical mapping of nucleosome positions in vitro

If the reaction is also supplied with a reducing agent (e.g. ascorbic acid or DTT), the iron III product can be recycled to iron II so that the iron/chelator complex acts as a catalytic centre (Figure 2). Generating multiple hydroxyl radicals per molecule