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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
are subbed with poly-lysine on the day of use: 50 µl of poly-lysine solution is allowed to settle on slides for 10-20 min; excess solution is wiped off and slides are baked at 60oC for 10 min. II. PROCEDURE A. Overview. A mixed population
Preparation of Poly A+ RNA and Northern Analysis
RNA, multiply OD260 value times 40 (1 OD260 is 40 µg/ml). For storage, precipitate mRNA with 10 µl of Glycogen solution, 40 µl of potassium acetate solution and 1 ml of -20°C 95% EtOH. Store at -70°C. 5) For Northern analysis, have heating block
for large-scale purification (>25 µg) of poly(A)+ RNAextracted from mammalian cells. Typically, between 1% and 10% of the RNA applied to the oligo(dT) column is recovered as poly(A)+ RNA. Because the method can be frustratingly slow, it is not recommended
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