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上海玉博生物科技有限公司
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文献和实验Procedures for In Vitro Production of Bovine Embryos
of embryos/oocytes placed initially in the microdrops. Return plates to the incubator.DAY 5 AFTER IVF - ADDITION OF SERUM (OPTIONAL)1. Place aliquots of heat-inactivated fetal calf serum ( stock 24 ) in the incubator ~1-2 h before adding
in Schneider''s medium from Sigma] 50 ml Fetal Bovine Serum (JRH #12103-78P) - Heat Inactivated (aliquoted in -20) 5 ml 1:100 Penicillin-Streptomycin (Gibco #15070-063) (aliquoted in -20) Sterile filter (0.2 µ) Store at 4°C - do not freeze B. ML-DmBG2
果蝇细胞的培养实验方法(Culturing Drosophila cells)
) ***[S2R+ and Kc cells will not grow in Schneider's medium from Sigma] 50 ml Fetal Bovine Serum (JRH #12103-78P) - Heat Inactivated (aliquoted in -20) 5 ml 1:100 Penicillin-Streptomycin (Gibco #15070-063) (aliquoted in -20) Sterile filter
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