gibco特约代理商|16140071 Fetal Bovi

The Under-Agarose Migration Assay

20% (v/v) bovine calf serum (BCS), heat inactivated or 1% (w/v) bovine serum albumin (BSA) Prepare in RPMI-1640 medium HBSS-Agarose    2.4% (w/v) agarose Prepare

Procedures for In Vitro Production of Bovine Embryos

of embryos/oocytes placed initially in the microdrops. Return plates to the incubator.DAY 5 AFTER IVF - ADDITION OF SERUM (OPTIONAL)1. Place aliquots of heat-inactivated fetal calf serum ( stock 24 ) in the incubator ~1-2 h before adding

Drosophila Cell Culture

in Schneider''s medium from Sigma] 50 ml Fetal Bovine Serum (JRH #12103-78P) - Heat Inactivated (aliquoted in -20) 5 ml 1:100 Penicillin-Streptomycin (Gibco #15070-063) (aliquoted in -20) Sterile filter (0.2 µ) Store at 4°C - do not freeze B. ML-DmBG2