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Medium-Throughput Production of Recombinant Human Proteins: Protein Production in E. coli

In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E . coli system for test expressing intracellular

Transformation of E. coli by Electroporation

  Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate

Quality-Control Testing of Mycoplasma Medium

, a complex growth medium is required for their culture. Although the exact requirements of most mycoplasma species are still incompletely defined, most can be grown in the laboratory in both liquid and solid agar media formulated to comprise as a minimum