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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
, during time series analyses or when screening transfor-mants for expression of a transformed gene. Under such circumstances, existing tech-niques are overly time consuming and yield more RNA than is necessary. The availability of a rapid RNA mini-prep
High-Resolution, IPG-Based, Mini Two-Dimensional Gel Electrophoresis
The reproducibility and resolving power of immobilized pH gradient (IPG) led two-dimensional gel electrophoresis (2-DE) technology to the heart of proteome projects. Despite the recent progress achieved in this field, 2-DE is still not widely
and yield by gel electrophoresis, although details are given for measuring DNA spectrophotometrically. The appendix lists alternative methods and larger scale purification protocols. Time scale Mini-preps involve an overnight growth step: Day
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