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文献和实验High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
SP‐A LS‐C17957 e Transforming growth factor alpha
Size‐Exclusion Chromatography with On‐Line Light Scattering
onto a Superdex 75 column (Pharmacia) with PBS as eluent at a flow rate of 0.5 ml/min. (A ) 1 mg/ml of native RNase; (B ) 1 mg/ml of RCM‐RNase; (C ) chromatograms in panels A and B are put onto the same scale for comparing the LS/RI ratios of native and reduced
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
antibody diluted in1% (w/v) milk in TBS-T (see Note 5 ) with constant shakingor rotating to ensure even coverage of the membrane with theantibody. This step is usually performed at 4°C overnight, butcan also be performed for shorter periods at room
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