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文献和实验Primary Cultures of Astrocytes from Fetal Bovine Brain
We describe here a method to obtain primary cell cultures from the cerebral cortex and the hypothalamus of bovine fetuses. We report how tissue origin, developmental stages, and culture medium conditions influence cell
Serum-Free and Feeder-Free Culture Conditions for Human Embryonic Stem Cells
. However, hESCs are usually grown in culture conditions using fetal bovine serum and mouse embryonic fibroblasts, two components that are not compatible with clinical applications. Consequently, the possibility to expand hESCs in serum-free and in feeder-free
Culture of Murine Megakaryocytes and Platelets from Fetal Liver and Bone Marrow
describes methods to establish a standard culture of murine MKs from fetal liver and bone marrow along with approaches to purify MKs using bovine serum albumin gradients and magnetic-activated cell sorting. These culture systems allow investigations of MK
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