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文献和实验Dynamic Flow Assay in a Parallel Plate Flow Chamber
of 10�/ml to coating region. Incubate 1 hr. 3. Aspirate off liquid, add 20� of 1% BSA to block for 1 hr. 4. Aspirate off BSA, add 20� of inhibitor for 1 hr. 5. Dishes are ready for use in flow assay. Flow Assay Using Parallel Plate Flow
V294.PART II，Chapter 3 划痕实验(Wound-Healing Assay)
of Individual Cells in the Wound-Healing Assay1. Prepare 60-mm culture dishes as described in step 1 (Subheading 3.1.) to beused to match wounds during image acquisitions. If time-lapse microscopy willbe used, this step is not necessary (see Note 4). 2. Plate
manufacturer’s recommendations. 3.4.2. b-Catenin Stabilization 1. One or 2 days prior to the assay, seed a 96-well plate with L-cells(see Note 9 ). Seeding one tenth of the cells from a confluent10-cm dish of L-cells into all 96 wells
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