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Standard Operating Procedures for T1-Phage Testing Assay

lawn, the lawn there will be lysed on incubation overnight.   II.   Materials and Equipments: DH10B (original glycerol stock) or DH10B cultured stock for phage test. DH10B-T1 phage infected cells (positive control).

TOP10 chemically competent cells

Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells

【资源】大肠杆菌的表型大全

expression of deoxyribose synthesis genes; permits uptake of large plasmids. See Hanahan D, US Patent 4,851,348. ***This has been called into question, as the DH10B genome sequence revealed that it is deoR+. See Durfee08, PMID 18245285. dnaJ