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Miniprep/Qiagen kit

  Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.

Procedure for GenEluteTM Plasmid Miniprep Kit（质粒小量提取）

upon the plasmid and culture density.For best yields,follow the instructions in the note below.Transfer the appropriate volume of the recombinant E.coli culture to a microcentrifuge tube and pellet cells at ≥12000 × g for 1 minute.Discard the supernatant. Note

Miniprep/Kit-free high-throughput protocol

.--Kathleen) and yields DNA that is clean enough for sequencing or for use as a PCR template. Protocol Transfer 1.5 mL of an overnight culture containing your plasmid to an eppendorf tube and spin at 5000 rpm for 5 min in a tabletop centrifuge