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文献和实验Side directed mutagenesis with 17kb plasmid-Molecular Bi
Hey everybody, I am working on a side-directed mutagenesis with the Stratagene℃ quickchange mutagenesis xl-kit. Here is my problem: My plasmid is 17kb big. Until now I have had no colonies on my plate. According to Stratagene℃ protocol, I used
Bifunctional Short Hairpin RNA (bi-shRNA): Design and Pathway to Clinical Application
a novel vector-driven bifunctional short hairpin RNA (bi-shRNA) technology that harnesses both cleavage-dependent and cleavage-independent RISC loading pathways to enhance knockdown potency. Consequent advantages provided by the bi-shRNA include a lower
Identification of Start Sites of Bi-Directional DNA Synthesis at Eukaryotic DNA Replication Origins
DNA replication is the key process for the conservation and transmission of genetic information in all living organisms. The bi-directional DNA replication does not start randomly along the genome but rather at precise chromosomal regions
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