biorbyt特约代理商|orb44730 TCR alph

In situ hybridisation to alpha satellite sequences (chromosome specific)

  Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA

Quantitating MHC Class I Ligand Production and Presentation Using TCR-Like Antibodies

cytometry application for determining the number of peptide–MHC complexes on the surface of cells grown in tissue culture that express an endogenous protein from which the peptide is derived. The procedure requires a monoclonal antibody with the ability

MHC 四聚体应用：TCR 样抗体研发

板，利用 ELISA 方法测定抗体亲和力，可达到高通量检测效果。Anna Sergeeva 等（2011）在研究人类急性髓细胞样白血病的 TCR 样抗体工作中，用 HLA-A2-PR1 多肽复合物免疫小鼠，分离小鼠脾脏细胞。培养单克隆细胞，制备抗体。将 MHC-PR1 多肽复合物包被于酶联板上，进行同量化 ELISA 筛选。在 2850 个克隆中，筛选到一个（8F4）具备亲和力的抗体。推荐阅读：1. TCR Mimic Monoclonal Antibody Targets a Specific